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ImmunoGen Inc
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STEMCELL Technologies Inc
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Image Search Results
Journal: Iranian Journal of Biotechnology
Article Title: Increased Expression Level of Human Blood Clotting Factor VIII Using NS0 Cell Line as a Host Cells
doi: 10.30498/ijb.2024.409915.3772
Figure Lengend Snippet: The morphological changes of the NS0 cells after transfection with the pcDNA3-BDD-FVIII. Cells were cultured at a density of 5×10 5 cells in 0.5 ml of medium in 24-well plates. A) Non-transfected NS0 cells, B) NS0 cells transfected with Lipofectamine 3000 and 2 μg plasmid DNA. The micrograph showed round cells with some variation in size and transparency.
Article Snippet: The
Techniques: Transfection, Cell Culture, Plasmid Preparation
Journal: Iranian Journal of Biotechnology
Article Title: Increased Expression Level of Human Blood Clotting Factor VIII Using NS0 Cell Line as a Host Cells
doi: 10.30498/ijb.2024.409915.3772
Figure Lengend Snippet: Measurement of the expressed recombinant hFVIII secreted in the cultured media of the transfected NS0 cells, by ELISA. The values are stated in ng.mL -1 . The concentration of rhFVIII in the culture media of treated NS0 cells with Mitomycin C (10 μg.mmL -1 - 2.5 hours), Lovastatin (20 μM-24 & 48 hours), and Metformin (20 mM-24 & 48 hours) after 48 h vs control (untreated-transfected NS0 cells). The error bar shows the standard deviation, and the results were obtained from 3 repetitions (Mean±SD). Statistically significant differences between groups using one-way ANOVA was accepted at p ≤ 0.05. ((ns) not significant, (*) p < 0.05, (**) p < 0.01, (***) p < 0.001 and (****) p < 0.0001).
Article Snippet: The
Techniques: Recombinant, Cell Culture, Transfection, Enzyme-linked Immunosorbent Assay, Concentration Assay, Control, Standard Deviation
Journal: mAbs
Article Title: Heterologous recombinant expression of non-originator NISTmAb
doi: 10.1080/19420862.2018.1486355
Figure Lengend Snippet: Measured molecular mass values for NISTmAb RM 8671 and non-originator lots NS0-59, NS0-60, and NS0-66.
Article Snippet:
Techniques:
Journal: mAbs
Article Title: Heterologous recombinant expression of non-originator NISTmAb
doi: 10.1080/19420862.2018.1486355
Figure Lengend Snippet: In vitro SPR binding data for NISTmAb RM 8671 and non-originator lots NS0-59, NS0-60, and NS0-66. (n = 3).
Article Snippet:
Techniques: In Vitro, Binding Assay